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Ct Values: How They Should be Assessed for SARS CoV 2 (COVID 19 PCR Testing vs. Rapid Antigen Tests)

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  • Ct Values: How They Should be Assessed for SARS CoV 2

    6:14

    Professor Michael Mina, MD explains how the viral dynamics of SARS-CoV-2, the infectious window, and how Ct values (cycle threshold values) should be interpreted from PCR SARS CoV 2 testing. And how Ct values on a logarithmic scale can be misinterpreted.

    See the full interview with Professor Michael Mina, MD on rapid antigen testing for COVID 19 here:

    Michael Mina, MD, Ph.D. is an Assistant Professor of Epidemiology, Immunology, and Infectious Diseases at Harvard T. H. Chan School of Public Health. Dr. Mina is also a core member of the Center for Communicable Disease Dynamics (CCDD) and an Associate Medical Director in Clinical Microbiology (molecular diagnostics) in the Department of Pathology at Brigham and Women’s Hospital, Harvard Medical School
    Dr. Mina’s full bio:

    The volunteer-based rapid test advocacy website that Dr. Mina is the director of: (a great place to learn more about rapid antigen tests for COVID-19)

    Interviewer:

    Kyle Allred
    Physician Assistant
    MedCram.com Producer and Co-Founder
    Contact: customers@medcram.com



    THE MEDCRAM WEBSITE:

    MedCram is a leader in online medical education. Our videos are utilized by thousands of clinicians and medical schools, PA programs, NP, RN, RT, and other professional programs. We offer over 60 medical topics and 50 hours of category 1 CME / CEs for medical professionals. See new topics and more about ct value in COVID 19.

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    MedCram videos are for education and NOT intended to replace recommendations from your doctor.

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    In this video, Michael Mina, MD illustrates Ct Values (Cycle threshold values), Rapid antigen COVID tests (including the availability of an antigen test for COVID 19) vs. PCR tests for coronavirus.
    #COVID19 #SARSCoV2 #CtValues

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  • Difference between RT-PCR, Antibody & Rapid Antigen test

    4:57

    While the RT-PCR test detects the viral RNA, antibody & rapid antigen tests detect antibodies & viral proteins respectively. Know how the three tests differ from each other and solve different purposes.

    RT-PCR test - Real-Time Polymerase Chain Reaction, commonly known as RT-PCR test, is the most preferred test for detecting COVID-19. RT-PCR test is a laboratory test that combines reverse transcription of RNA into DNA for the detection of the virus. An RT-PCR test uses nose or throat swabs for the detection of viruses.

    Antibody Test - Antibody Test determines if the patient had COVID-19 in the past and now has antibodies against the virus. Antibody testing is also known as serology testing and is usually done after full recovery from COVID-19.

    Rapid Antigen Test - A rapid antigen test is a diagnostic test suitable for point-of-care testing that directly detects the presence or absence of an antigen. It is commonly used for the detection of SARS-CoV-2, the virus that causes COVID-19. Turnaround time for rapid antigen test results is usually very quick and can be reported within 15-30 minutes.

    Watch the full video to know more details.
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    Click on below links to know more:
    Antibody test -

    Covid 19 test -
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    Related Videos:
    Know about COVID-19 vaccine in India -

    Antibody Testing -

    Difference between RT-PCR, Antibody & Rapid Antigen test (Hindi) -
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    #Covid19 #RapidAntigenTest #AntibodyTest #RTPCRTest #Covidtest #MetropolisLabs​

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  • At Home Rapid COVID 19 Tests and False Positives

    3:41

    Harvard professor and epidemiologist Michael Mina, MD responds to the idea that inexpensive at-home coronavirus tests would produce too many false positives to be useful as a screening tool to curb the pandemic. False positives occur when someone tests positive but is not infected with SARS-CoV-2 that causes COVID-19.

    See our previous MedCram videos that have featured Dr. Mina's research about COVID 19 testing and how quickly identifying individuals who are contagious (and who have high levels of SARS-CoV-2 virus) should be the primary focus.

    Dr. Mina, Kyle Allred, Dr. Seheult, and MedCram have no financial disclosures related to this topic. We have a keen interest in curbing this pandemic and safely re-opening schools and our economy.

    Michael Mina, MD Ph.D. is an Assistant Professor of Epidemiology, Immunology, and Infectious Diseases at Harvard T. H. Chan School of Public Health. Dr. Mina is also a core member of the Center for Communicable Disease Dynamics (CCDD) and an Associate Medical Director in Clinical Microbiology. Dr. Mina's bio is here:

    MORE VIDEOS AND RESOURCES ON AT-HOME COVID 19 SCREENING TESTS:

    Dr. Mina is the director (on a volunteer basis) of where you can get more info and streamlined ways to contact your governor and representatives in Congress about at-home COVID 19 screening

    A 3-minute explainer video on inexpensive at-home rapid screening tests by MedCram:

    A video summary and highlights of Dr. Mina's research and ideas in our MedCram COVID 19 Update 98:

    A 5 minute summary with Dr. Mina of some key differences between COVID-19 antigen tests with rapid results:

    A short explainer video on daily quick tests produced by Dr. Allen:

    Summary Article Featured in the Atlantic about Inexpensive Rapid Testing:

    Dr. Mina's research paper: Test sensitivity is secondary to frequency and turnaround time for COVID-19 surveillance (Pre-print)

    TWiV 640: Test often, fast turnaround, interview with Michael Mina:


    ------------------------------------------------------
    Video produced by Kyle Allred, Co-Founder of
    Contact Kyle or MedCram by emailing: customers@medcram.com

    #COVID19 #rapidtestsnow #dailyquicktest

  • rt pcr test for covid 19 | what is ct value in coronavirus rt pcr test?

    31:49

    rt pcr test for covid 19 | what is ct value in coronavirus rt pcr test? - This lecture explains about the details of rt pcr test and how rt pcr test is used to identify the coronavirus infection in human. This lecture explains the steps of reverse transcriptase pcr reaction and the application of rt pcr reaction to detect covid 19 disease. So, if you have covid 19 symptoms, go for rt pcr test to find out the novel coronavirus infection. stay tuned to know the following answers -
    what is covid 19 test?
    how coronavirus testing is done?
    what is rt pcr test?
    steps of rt pcr test?
    what is ct value of rt pcr?
    application of rt pcr in covid 19 diagnosis?

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    Thank you for watching the video lecture on the rt pcr test for covid 19 | what is ct value in coronavirus rt pcr test?.

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  • COVID Diagnosis with PCR | Misinterpreting results | Cycle threshold explained

    11:16

    #PCR testing to diagnose #COVID may be resulting in wrong diagnoses, unnecessary scares about #reinfection, and wasted resources. This video explains why RT-PCR tests, which are the gold standard for diagnostic testing, may be hindering our fight against COVID-19; how, after seven months, we still aren’t doing testing right; and what experts believe needs to change. Most importantly, we explore the role of the cycle threshold in all of this. Check out what a Ct is and why experts are calling for greater reporting of the Ct in results and changes to its threshold in laboratory protocols.

    By popular demand (even though it is not the subject of this video), I have been asked to provide links to studies in which SARS-CoV-2 was isolated in cell culture, visualized by electron microscopy, reacted with antibodies, genetically sequenced, and demonstrated to fulfill Koch's postulates. Those links are at the very bottom.

    Check out my channel for all things public health: @Anthony Kuster
    _____________________________________
    ***Links to the underlying research and articles referenced in this video below:

    Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to May 2020 |


    63 days detection of SARS-CoV-2 RNA from a recovered patient in Southern Italy: A Case Report |


    Absent immune response to SARS-CoV-2 in a 3-month recurrence of coronavirus disease 2019 (COVID-19) case |


    SARS-CoV-2 RNA Persistence in Naso-Pharyngeal Swabs |


    Long-Haulers Are Redefining COVID-19 |


    COVID-19 re-infection by a phylogenetically distinct SARS-coronavirus-2 strain confirmed by whole genome sequencing |


    To Interpret the SARS-CoV-2 Test, Consider the Cycle Threshold Value |


    Your Coronavirus Test Is Positive. Maybe It Shouldn’t Be. |


    Testing Dilemmas: Post negative, positive SARS-CoV-2 RT-PCR - is it a reinfection? |


    Challenges and Controversies to Testing for COVID-19 |


    ‘It’s Kitchen Sink Time’: Fast, Less-Accurate Coronavirus Tests May Be Good Enough |


    ______________________________
    ****By popular demand, isolation and purification of SARS-CoV-2 and fulfillment of Koch's postulates (keep in mind, though, that Koch made his postulates to describe cholera and TB and was written in the 1800s before viruses were discovered):

    What does it mean to isolate and purify a virus?

    A virologist's summary and review of relevant literature:


    The pathogenicity of SARS-CoV-2 in hACE2 transgenic mice (fulfillment of Koch's postulates) |

    Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2 |

    Virus Isolation from the First Patient with SARS-CoV-2 in Korea |

    Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient |

    SARS-CoV-2 infection diagnosed only by cell culture isolation before the local outbreak in an Italian seven-week-old suckling baby |

    Isolation of infectious SARS-CoV-2 from urine of a COVID-19 patient |

  • Reliable, rapid PCR test for the detection of SARS-CoV-2 in 30 minutes

    1:41

    The Thermo Fisher Scientific Accula™ System is a rapid diagnostic platform that combines the accuracy of RT-PCR with the simplicity, convenience, and procedural familiarity of traditional rapid immunoassays. The Accula™ SARS-CoV-2 Test has received an Emergency Use Authorization (EUA) from the FDA for the detection of SARS-CoV-2 in Clinical Laboratory Improvement Amendments (CLIA)–waived environments. Designed for use with the palm-sized Accula™ Dock, the Accula SARS-CoV-2 Test provides reliable, qualitative results in approximately 30 minutes.

    The Accula SARS-CoV-2 Test and Accula Dock Feature:

    • Accurate PCR technology—100% positive agreement (PPA) and negative agreement (NPA) with an EUA-authorized RT-PCR SARS-CoV-2 test comparator
    • Simple sample collection—prepare and load nasal swab samples in seconds
    • Fast results—qualitative, rapid PCR-based test provides visual results in 30 minutes
    • Easy storage—reagents stored at room temperature (15°C–30°C, 59°F–86°F); eluted samples in Accula™ buffer may be kept at room temperature for up to 2 hours or refrigerated at 2°C–8°C and tested within 24 hours from the time of elution
    • Compact design—dock fits in the palm of your hand; ideal fit for satellite locations and clinical settings

    Learn more:


    Accula™ SARS-CoV-2 Test


    Accula™ Dock


    Accula™ SARS-CoV-2 Control Kit


    Gold-standard RT-PCR detection of SARS-CoV-2 at rapid speed: a solution for asymptomatic screening


    Reliable, rapid PCR test for the detection of SARS-CoV-2 in ~30 minutes


    #RapidPCR #CovidTesting #Accula #Covid19Testing

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  • CT-Scans vs. RT-PCR vs. Rapid Antigen: Which test to go for and when

    45:39

    Indian Council of Medical Research (ICMR) issued a new Covid-19 testing advisory on Tuesday that recommends using more rapid antigen tests in the wake of the massive surge in cases that has put huge pressure on India’s healthcare system. ICMR has also suggested reducing RT-PCR tests to take the load off the existing laboratories. CT-Scans vs. RT-PCR vs. Rapid Antigen: Which test to do and when. Top Doctors Dr MS Kanwar (Apollo Hospital) & Dr Amit Thadani (Niramaya Hospital), join us to address your queries at 6pm. Do start sending in your questions.

  • Understanding Different COVID-19 Tests, Animation

    3:01

    Coronavirus testing: diagnostic tests - rapid antigen test versus molecular tests (nucleic acid amplification test, NAAT) for viral RNA (RT-PCR, LAMP,…); and antibody (serology) test for past infection. Nasal swab versus nasopharyngeal swabs, false positive and false negative rates. Accuracy of tests, sensitivity, detection limits, turnaround time and relative costs.
    This video is available for instant download licensing here:
    ©Alila Medical Media. All rights reserved.
    Support us on Patreon and get early access to videos and free image downloads: patreon.com/AlilaMedicalMedia
    All images/videos by Alila Medical Media are for information purposes ONLY and are NOT intended to replace professional medical advice, diagnosis or treatment. Always seek the advice of a qualified healthcare provider with any questions you may have regarding a medical condition.
    There are 2 major types of COVID-19 tests: diagnostic tests for active infection, and antibody tests for past infection.
    Diagnostic tests look for components of the virus in a sample taken from the nasal cavity, throat, or saliva. Sample taken from the nasopharynx, the upper part of the throat behind the nose, is preferred when higher accuracy is required.
    There are 2 types of diagnostic tests: molecular tests detecting viral RNA, and antigen tests (best for pre-travel screening) detecting viral proteins.
    Antigen tests use a technology similar to that of a pregnancy test. Some are made available as at-home test kits. The test is fast and less expensive, but is less sensitive. Antigen test gives positive results only with high viral loads, when the person is near the peak of infection, so it’s more likely to miss an active infection. In other words, the rate of false negative – a test that says you don’t have the virus when you actually do, is high. Symptomatic patients who test negative with rapid antigen test must be confirmed with a more sensitive molecular test. On the other hand, positive results are highly accurate, but false positive – a test that says you have the virus when you actually don’t, can still happen, most commonly due to errors in sample handling.
    Molecular tests detect viral RNA. They are also called nucleic acid amplification tests, NAAT, because they amplify viral nucleic acids until there are detectable levels. Different tests are based on different technologies, with polymerase chain reaction, PCR, being just one of them. PCR is the gold standard for diagnostic testing but it requires specific equipment and takes longer to deliver results.
    Molecular tests are much more sensitive than rapid antigen tests, but they can still produce false-negative results early in the infection. On the other hand, the high sensitivity may sometimes pick up the low viral load in a patient who has recovered and is no longer contagious. Positive results are highly accurate, most false positives are due to lab contamination or other errors with sample handling.
    Antibody tests, also called serology tests, detect antibodies that the body produced in response to the infection. A blood sample is taken for this test. Because antibodies can take a couple of weeks to develop and may stay in the blood for weeks or months after recovery, a positive test result only proves that the person has been exposed to the virus. It gives no indication about active infection and should not be used to diagnose COVID-19.

  • What is RT PCR test for COVID 19? What is Ct Value? What is CT Score?

    27:33

    The COVID-19 RT-PCR test is a real-time reverse transcription polymerase chain reaction (rRT-PCR) test for the qualitative detection of nucleic acid from SARS-CoV-2 in upper and lower respiratory specimens (such as nasopharyngeal or oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate) collected from individuals suspected of COVID-19 by their healthcare provider (HCP), as well as upper respiratory specimens (such as nasopharyngeal or oropharyngeal swabs, nasal swabs, or mid-turbinate swabs) collected from any individual, including for testing of individuals without symptoms or other reasons to suspect COVID-19 infection.
    All patients with a CT value of equal to or less than 35 may be considered positive, while those with a CT value greater than 35 may be considered negative. All samples with a CT value equal to or less than 35, which is seen as a poor sigmoidal curve, should be essentially retested. The CT severity score index is a scoring system used to assess the lung changes and involvement by COVID-19 based on approximate estimation of pulmonary involved areas. Each of the five lung lobes has been visually scored and given a score from 1 to 5: 1 representing less than 5% lobar involvement.
    Join this channel to get access to perks:

  • x
  • What Is The Difference Among RT-PCR, Antibody & Antigen Tests Of Coronavirus? | NewsMo

    4:26

    With India crossing the million mark in Covid cases, there's a need to speed up the testing process. So, what are the required tests? Watch the video to know about different tests done for Coronavirus.
    #Newsmo #covid19 #coronavirus #coronavirustesting


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  • Rapid Coronavirus Testing - At HOME with Dr. Michael Mina

    1:7:07

    Michael Mina, MD, PhD responds to questions about how cheap (approx. $1), at-home, COVID-19 tests (results in 15 minutes) could be utilized to dramatically slow the spread of this pandemic (and open up schools etc. in a faster and safer way).

    Dr. Mina is an Assistant Professor of Epidemiology at the Harvard T.H Chan School of Public Health and a core member of the Center for Communicable Disease Dynamics. Dr. Mina's research has demonstrated that the sensitivity of these simple daily quick tests (the technology already exists) is high enough to detect the vast majority of infectious COVID-19 and could be utilized frequently at home with a nasal or saliva sample.


    LINKS / REFERENCES:

    Please watch MedCram Update 98 for context on Dr. Mina's research and ideas:
    We read your comments (from Update 98, and at MedCram.com) for COVID testing questions to ask Dr. Mina.

    Michael Mina, MD, Ph.D.'s bio:

    Dr. Mina's New York Times article about a new COVID-19 testing paradigm:

    Dr. Mina's research paper: Test sensitivity is secondary to frequency and turnaround time for COVID-19 surveillance (Pre-print)

    Article featuring Dr. Mina's ideas published in Harvard Magazine on August 3, 2020

    TWiV 640: Test often, fast turnaround, with Michael Mina:

    Follow Dr. Mina on Twitter:

    Thanks for joining the event and please spread the word to friends and colleagues.

    A big thanks to Dr. Michael Mina for spending an hour with us.

    GET INVOLVED

    If you're seeking templates and streamlined ways to contact your governor and representatives in Congress about daily quick tests / rapid COVID 19 testing please visit the volunteer-based website that Dr. Mina is the director of.

    #RapidTestsNow #COVID19 #DailyQuickTest

  • Are At Home COVID 19 Antigen Tests Accurate Enough?

    2:04

    Harvard professor and epidemiologist Dr. Michael Mina responds to the question of accuracy and sensitivity related to inexpensive paper antigen tests. Learn what influences accuracy is why it is relative to the goal of the test (screening for contagious COVID-19 vs testing for low virus levels that are often discovered on the tail end of infection).

    See our previous MedCram videos that have featured Dr. Mina's research about COVID 19 testing and how quickly identifying individuals who are contagious (and who have high levels of SARS-CoV-2 virus) should be the primary focus.

    Dr. Mina, Kyle Allred, Dr. Seheult, and MedCram have no financial disclosures related to this topic. We have a keen interest in curbing this pandemic and safely re-opening schools and our economy.

    Michael Mina, MD Ph.D. is an Assistant Professor of Epidemiology, Immunology, and Infectious Diseases at Harvard T. H. Chan School of Public Health. Dr. Mina is also a core member of the Center for Communicable Disease Dynamics (CCDD) and an Associate Medical Director in Clinical Microbiology. Dr. Mina's bio is here:

    MORE VIDEOS AND RESOURCES ON AT-HOME COVID 19 SCREENING TESTS:

    Dr. Mina is the director (on a volunteer basis) of where you can get more info and streamlined ways to contact your governor and representatives in Congress about at-home COVID 19 screening

    A 3-minute explainer video on inexpensive at-home rapid screening tests by MedCram:

    A video summary and highlights of Dr. Mina's research and ideas in our MedCram COVID 19 Update 98:

    A 5 minute summary with Dr. Mina of some key differences between COVID-19 antigen tests with rapid results:

    A short explainer video on daily quick tests produced by Dr. Allen:

    Summary Article Featured in the Atlantic about Inexpensive Rapid Testing:

    Dr. Mina's research paper: Test sensitivity is secondary to frequency and turnaround time for COVID-19 surveillance (Pre-print)

    TWiV 640: Test often, fast turnaround, interview with Michael Mina:

    Visit MedCram.com for over 100 coronavirus pandemic updates including sensitivity vs specificity, covid 19 tests, diagnosis and treatment, and many more topics.

    ------------------------------------------------------
    Video produced by Kyle Allred, Co-Founder of
    Contact Kyle or MedCram by emailing: customers@medcram.com

    #COVID19 #rapidtestsnow #dailyquicktest

  • Coronavirus Update 98: Rapid COVID 19 Antigen Testing at Home - A Possible Breakthrough

    17:06

    Professor Roger Seheult, MD discusses the current COVID-19 testing strategy in the United States that the CDC estimates picks up less than 10% of infections. Dr. Seheult of MedCram.com features snippets from a recent This Week in Virology (Episode 640) with Dr. Michael Mina of Harvard who explains that the technology exists for at home, inexpensive, self-administered, paper COVID-19 antigen tests that are likely accurate enough to pick up the vast majority of infectious cases of COVID-19. And how lower sensitivity of inexpensive tests may be ideal for a screening test that is performed with high frequency. Utilization of these daily quick tests may provide the optimal path forward to curtail the growth of COVID-19 and open schools and our economy in a much safer way. (This video was recorded July 20, 2020).

    LINKS / REFERENCES:

    See a MedCram Q/A follow up to this video with Dr. Michael Mina:
    Dr. Mina speaks more about COVID-19 testing responds to questions from the comment section below.

    This Week in Virology Episode 640 |

    NEJM |

    NYU Study on Abbott ID Now |

    New York Times |

    Test Sensitivity is Secondary to Frequency (Dr. Mina) |

    Abbott ID Now |

    CNN Test Costs |

    Johns Hopkins Tracker |

    Live Science |

    Korean CDC |

    Washington Post |

    Reuters |

    THE MEDCRAM WEBSITE:

    Visit us for videos on over 60 medical topics and CME / CEs for medical professionals:


    SUBSCRIBE TO THE MEDCRAM YOUTUBE CHANNEL:


    Get notified of new videos by hitting the bell icon:


    PREVIOUS COVID-19 UPDATES:

    Please visit MedCram.com for the full series:
    - Coronavirus Pandemic Update 97: Vitamin D & COVID-19 Immunity, The Endothelium, & Deficiencies
    - Coronavirus Pandemic Update 96: RNA Vaccine; Ivermectin; von Willebrand Factor and COVID-19
    - Coronavirus Pandemic Update 95: Widespread Clotting on Autopsy; New COVID-19 Prognostic Data
    - Coronavirus Pandemic Update 94: Inhaled Steroids COVID-19 Treatment; New Pneumonia in Kazakhstan?
    - Coronavirus Pandemic Update 93: Antibodies, Immunity, & Prevalence of COVID-19 - New Data from Spain
    - Coronavirus Pandemic Update 92: Blood Clots & COVID-19 - New Research & Potential Role of NAC
    - Coronavirus Pandemic Update 91: Remdesivir Pricing & Disparities in Drug Availability
    - Coronavirus Pandemic Update 90: Assess The Quality of COVID-19 Info With A Validated Research Tool
    - Coronavirus Pandemic Update 89: COVID 19 Infections Rising in Many States; Dexamethasone Cautions

    All coronavirus updates are at MedCram.com (including the prevention, treatment, vaccine developments, rapid COVID-19 testing, Daily quick tests, coronavirus antigen tests, coronavirus misinformation, and more). We offer many other medical topics at our website.


    MEDCRAM WORKS WITH MEDICAL PROGRAMS AND HOSPITALS:

    MedCram offers group discounts for students and a variety of medical programs, hospitals, and other institutions. Contact us at customers@medcram.com if you are interested.


    MEDIA CONTACT:

    Roger Seheult, MD
    Board Certified in Internal Medicine, Pulmonary Disease, Critical Care, and Sleep Medicine.
    Associate Professor at the University of California, Riverside School of Medicine

    Media Contact: customers@medcram.com
    Media contact info:

    Video Produced by Kyle Allred

    FOLLOW US ON SOCIAL MEDIA:






    DISCLAIMER:

    MedCram medical videos are for medical education and exam preparation, and NOT intended to replace recommendations from your doctor.
    #RapidTestsNow #COVID19 #DailyQuickTest

  • COVID 19 Update || Rapid Antigen Test vs PCR Test || What you need to know about the COVID testing

    12:05

    #COVID #coronavirus #RapidantigenTest #COVIDTesting #PCRTesting

    Hi everyone! Welcome back to this weeks' COVID- update.
    Schools have started, and many will be starting very soon. Words are buzzing around COVID testing. In this video, I am going to explain the key important facts of different COVID testing. It is important for everyone to understand the advantage and limitations of each type of COVID diagnostic test.

    To learn more:





  • What CT Value Means in a COVID Test | NewsMo

    3:00

    As COVID's second wave continues to ramp up at full pace, the emphasis still remains on widespread testing. We all are familiar with different types of tests, but what we don't know is the meaning of CT level in our RT-PCR reports. So, watch to find out.

    #Covid #CT #NewsMo #IndiaToday

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  • CT Value in RT-PCR: Lower Value - Higher Viral Load | Different from CT Score

    4:44

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    Short for cycle threshold, Ct is a value that emerges during RT-PCR tests, the gold standard for detection of the SARS-CoV-2 coronavirus. In an RT-PCR test, RNA is extracted from the swab collected from the patient. It is then converted into DNA, which is then amplified. Amplification refers to the process of creating multiple copies of the genetic material — in this case, DNA. This improves the ability of the test to detect the presence of virus. Amplification takes place through a series of cycles — one copy becomes two, two becomes four, and so on — and it is after multiple cycles that a detectable amount of virus is produced.

  • COVID-19 Rapid Tests Demo & Q/A with E25Bio Co-Founder

    23:38

    Simple and cheap COVID-19 tests that provide rapid results (15 min.) have been called a possible game-changer to curb the pandemic and allow schools and stores to open safely. Our guest today is Bobby Brooke Herrera, Ph.D. - the Co-Founder of a small company based in Massachusetts called E25Bio. Back on February 26, 2020, E25Bio had its inexpensive COVID-19 antigen test developed, but his company has run into numerous hurdles - lack of guidance and reasonable standards from the FDA, limited federal investment into public health testing strategies, and challenging pathways to production and distribution.

    Dr. Herrera gives a demo of their simple test that requires a simple nasal swab, plastic tube, a paper strip, and no additional processing. He also discusses how tests like this could be distributed and utilized to quickly curtail the spread of COVID-19. (This video was recorded August 12, 2020).

    LINKS / REFERENCES:

    More about Dr. Bobby Brooke Herrera - Co-Founder of E25Bio |

    MedCram Update 98 for context on rapid daily quick tests and Dr. Mina's research |

    5-Minute Sharable Video About At-Home Rapid Tests |

    Boston Globe Article about E25Bio |

    THE MEDCRAM WEBSITE:

    Visit us for videos on over 60 medical topics and CME / CEs for medical professionals:


    SUBSCRIBE TO THE MEDCRAM YOUTUBE CHANNEL:


    Get notified of new videos by hitting the bell icon:


    PREVIOUS COVID-19 UPDATES:

    Please visit MedCram.com for the full series:
    - COVID-19 Pandemic Update 101: T Cell Immunity vs. Antibodies & Prior Exposure to Other Coronaviruses
    - At Home, Cheap, COVID-19 Tests with Results in 15 Minutes: How to Fix Testing with Dr. Michael Mina
    - Coronavirus Pandemic Update 100: Interferon Beta COVID-19 Treatment, and a Retrospective
    - Coronavirus Pandemic Update 99: Long Haulers - Lingering & Long-Term Symptoms After COVID-19
    - Coronavirus Pandemic Update 98: At Home COVID-19 Testing - A Possible Breakthrough
    - Coronavirus Pandemic Update 97: Vitamin D & COVID-19 Immunity, The Endothelium, & Deficiencies
    - Coronavirus Pandemic Update 96: RNA Vaccine; Ivermectin; von Willebrand Factor and COVID-19
    - Coronavirus Pandemic Update 95: Widespread Clotting on Autopsy; New COVID-19 Prognostic Data
    - Coronavirus Pandemic Update 94: Inhaled Steroids COVID-19 Treatment; New Pneumonia in Kazakhstan?
    - Coronavirus Pandemic Update 93: Antibodies, Immunity, & Prevalence of COVID-19 - New Data from Spain
    - Coronavirus Pandemic Update 92: Blood Clots & COVID-19 - New Research & Potential Role of NAC
    - Coronavirus Pandemic Update 91: Remdesivir Pricing & Disparities in Drug Availability

    All coronavirus updates are at MedCram.com (including more on vaccine developments, rapid COVID-19 testing, paper COVID 19 antigen tests, COVID 19 antibody tests, coronavirus daily quick tests, coronavirus news, COVID test, peer-reviewed research, and more).
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    #COVID19 #RapidTestsNow #DailyQuickTest

  • I tested positive for SARS-CoV-2

    14:23

    Recently I tested positive for SARS-CoV-2. In this video I explain how the PCR test was done, what the Ct value that I received means, and why I was not worried about the result.

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  • Describing antigen testing for COVID 19

    9:57

    Antigen testing offers a cheaper and faster method of diagnosing COVID-19 compared to RT-PCR–but it comes with a significant limitation. In this video from our COVID Mini: Diagnostics course, we look into how this test works and consider the benefits and drawbacks of integrating it into the COVID-19 diagnostic process.

    Continue your understanding of this deadly disease through our COVID Mini: Diagnostics course.

    Check out our course library and register for a free trial account:

    Please Note: Medmastery's videos, medical lectures, medical illustrations, and medical animations are for medical education and exam preparation purposes, and not intended to replace recommendations by your doctor or health care provider.

    #medmastery #coronavirus #COVID19 #sarscov2 #coronaviruschina #coronavirustruth #WHO #wuhan #infection #pandemic #publichealth

  • Rapid Antigen Test for Covid-19 . Principle, How it works?

    6:45

    What rapid Antigen test is? What is its principle? How the test is done? How it is different from antibody and RT-PCR tests.

    ***Support us*** by donating to our PayPal. This will motivate us creating more such content for you.
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    Recommended videos:
    1. RT-PCR test for Covid 19:


    2. How Covid 19 rapid antibody kit works:

    3. How vaccine works

  • x
  • At Home COVID 19 Antigen Testing and Vaccine Update with Harvard Professor Michael Mina, MD

    1:1:37

    Professor Michael Mina, MD answers key questions about at-home COVID 19 testing and coronavirus vaccines:
    0:00 We need a new testing strategy and at home rapid tests have advantages to PCR
    2:49 What other countries are using rapid antigen paper COVID-19 tests?
    3:25 Rapid decline of COVID-19 spread in Slovakia with paper antigen COVID 19 tests
    3:48 At home coronavirus tests are an intervention most people actually want (unlike distancing, shutdowns, etc.)
    6:21 FDA barrier to rapid at home COVID 19 tests
    6:40 The sensitivity of at-home tests to capture someone who is infectious with COVID 19 is 95-99%
    6:53 Simple ways to address false positives
    7:45 Manufacturing capacity needed for 10-20 million paper strip tests per day
    8:17 Dr. Mina’s discussions with various US leaders and elected officials about at home tests
    8:41 Dr. Fauci on at home coronavirus tests
    9:21 Who’s job is it to make rapid at home tests a reality?
    12:07 FDA approves the first at home SARS-CoV-2 test (made by Lucira), but will it be useful?
    15:35 New York Times article with a misleading title about rapid antigen tests
    16:09 PCR vs antigen tests are useful for completely different purposes
    20:32 Capturing the contagious window of COVID-19 with testing (the viral dynamics and the Ct values of SARS CoV 2)
    28:14 CDC estimates that over half of COVID-19 spread is asymptotic or pre-symptomatic
    31:05 Are any test manufactures collaborating on COVID-19 testing in the US (and using confirmatory tests)?
    36:17 What rapid COVID-19 have been approved in the US or outside the US that are suitable for a self test?
    36:46 Abbott Binax Now Test
    37:11 Abbott Panbio Test
    37:48 Roche SD Biosensor COVID-19 Test
    38:00 AccessBio Antigen Test
    38:32 E25Bio Rapid COVID-19 Test
    40:18 What entity should oversee at home COVID-19 screening tools? The FDA? CDC? a new entity?
    45:52 The (relatively small) expense of at home COVID-19 testing
    48:53 Practical testing tips for the testing services we have available now?
    54:05 Common misconceptions about rapid COVID-19 testing
    56:15 Will a viable coronavirus vaccine diminish the need for at home testing?
    57:09 Concerns about COVID 19 vaccine efficacy including plasmablast response
    1:00:00 Easier to adjust a paper test than a vaccine
    1:01:10 Parting words and how to learn more about rapid tests

    Michael Mina, MD, Ph.D. is an Assistant Professor of Epidemiology, Immunology, and Infectious Diseases at Harvard T. H. Chan School of Public Health. Dr. Mina is also a core member of the Center for Communicable Disease Dynamics (CCDD) and an Assoc. Medical Director in Clinical Microbiology in the Department of Pathology at Brigham and Women’s Hospital, Harvard Medical School
    Dr. Mina’s full bio:

    The volunteer-based rapid test advocacy website that Dr. Mina is the director of:
    (a great place to learn more about rapid at-home tests or help advocate for them)

    Interviewer:

    Kyle Allred
    Physician Assistant
    MedCram.com Producer and Co-Founder
    Contact: customers@medcram.com


    LINKS FROM THIS INTERVIEW:

    Science Advances: Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening (Dr. Mina is a co-author) |

    Economic Benefits of COVID-19 Screening Tests (Dr. Mina is a co-author) |

    Time: How We Can Stop the Spread of COVID-19 By Christmas (by Dr. Michael Mina) |

    New York Times: A Rapid Virus Test Falters in People Without Symptoms, Study Finds |

    Worldometer: Slovakia COVID-19 Data:

    CDC |

    FDA |

    Previous MedCram Interview with Dr. Michael Mina on COVID-19Testing:

    Fast Company: Slovakia tested almost everyone in the country and ‘broke the curve’ |

    Dr. Mina's Twitter |

    TWiV 640 |

    MedCram: COVID-19 Rapid Tests Demo & Q/A with E25Bio Co-Founder |


    THE MEDCRAM WEBSITE:

    MedCram is a leader in online medical education. Our videos are utilized by thousands of clinicians and medical schools, PA programs, NP, RN, RT, and other professional programs. We offer over 60 medical topics and 50 hours of CME/CEs for medical professionals.

    Learn more:

    MedCram videos are for education and NOT intended to replace recommendations from your doctor.
    #COVID19 #SARSCoV2 #RapidTestsNow

  • RT PCR test for coronavirus/ Covid-19. How it works? Made Easy.

    11:04

    Most exhaustive information about RT-PCR, types, working principle, requirements, advantages, applications will be covered in this video with a special emphasis on Covid-19 detection.


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    Recommended videos:
    1. Rapid Antigen Test


    2. Rapid antibody test

    3. How vaccine works

  • Ct values in the context of multiple RT-PCR testing for SARS-CoV-2 - Video abstract ID 282962

    2:45

    Video abstract of a short report paper Cycle threshold values in the context of multiple RT-PCR testing for SARS-CoV-2 published in the open access journal Risk Management and Healthcare Policy by Daniel Romero-Alvarez, Daniel Garzon-Chavez, Franklin Espinosa et al.

    Purpose: Discharge or follow up of confirmed coronavirus disease 2019 (COVID-19) cases depend on accurate interpretation of RT-PCR. Currently, positive/negative interpretations are based on amplification instead of quantification of cycle threshold (Ct) values, which could be used as proxies of patient infectiousness. Here, we measured Ct values in hospitalized confirmed COVID-19 patients at different times and its implications in diagnosis and follow up.

    Patients and Methods: Observational study between March 17th-May 12th, 2020 using multiple RT-PCR testing. A cohort of 118 Hispanic hospitalized patients with confirmed COVID-19 diagnosis in a reference hospital in Quito, Ecuador. Multiple RT-PCR tests were performed using deep nasal swab samples and the assessment of SARS-CoV-2 genes N, RdRP, and E.

    Results: Patients’ median age was of 49 years (range: 24– 91) with a male majority (62.7%). We found increasing levels of Ct values in time, with a mean Ct value of 29.13 (n = 61, standard deviation (sd) = 5.55) for the first test and 34.38 (n = 60, sd = 4), 35.52 (n = 20, sd = 2.85), and 36.12 (n = 6, sd = 3.28), for the second, third, and fourth tests, respectively. Time to RT-PCR lack of amplification for all tests was of 34 days while time to RT-PCR Ct values > 33 was of 30 days.

    Conclusion: Cycle thresholds can potentially be used to improve diagnosis, management and control. We found that turnover time for negativity can be large for hospitalized patients and that 11% cases persisted with infectious Ct values for more time than the current isolation recommendations.

    Read the full paper here:

  • COVID-19 Testing Explainer: Sensitivity, Specificity, and Predictive Values

    5:55

    Diagnostic sensitivity, specificity, and predictive values are all important metrics that are used to evaluate the performance of laboratory tests, and that can help you to gauge how reliable a COVID-19 test result is in your local patient population.

  • Positive PCR Test. Is It Really Positive?

    48:31

    Positive PCR Test. Is It Really Positive?

    More lectures on drbeen.com

    Looking to support my educational work? Donate here:



    Today we will discuss the CDC recommendation for the utility of the PCR tests. We will also discuss the recommendations by the epidemiologists. And, finally I will provide my commentary.

    CDCs Thoughts on PCR



    Impacts contact tracing.
    Begs the question; if someone had coronavirus fragments. Did he/she develop the immunity? That means, before blindly reducing the cycle threshold. We should have done a trial of testing for PCR with Antibodies at both cycle thresholds.

    Your Coronavirus Test Is Positive. Maybe It Shouldn’t Be.


    CDCs Clarification


    Faster tests are better


    A possible fast test


    CDC article about PCR

  • Antigen Tests with Accurate and Rapid Results: A Home Test for Coronavirus may Curb Pandemic

    2:27

    A home test for coronavirus (SARS-CoV-2) has been advocated by a variety of infectious disease experts including Harvard epidemiologist Professor Michael Mina, MD.

    This draft video was accidentally posted. Please watch the updated/final version here:

  • 65% Rapid Antigen Test Results Were False Negatives | V6 News

    1:49

    More than half the symptomatic patients who tested negative for Covid-19 in an antigen test later came positive with the realtime PCR method, reveal findings from two major civic laboratories. This underscores the high rate of false negatives in rapid antigen testing, which started this month under BMC’s ‘Mission Universal Testing’ plan.For instance, 60% of 538 symptomatic who tested negative in antigen tests at Nair Hospital, came out positive when retested under RTPCR. At Kasturba, the finding was reversed for 65% (28) of the 43 patients.

    పీపీఈ కిట్లతో పెండ్లి భోజనాలు,నాటుకోడి ఫుల్లు గిరాకీ


    జోరుగా వరినాట్లు


    అప్పడాలతో కరోనా పోతదా...



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    #RapidAntigenTests #COVID19 #Coronavirus
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  • RT-PCR & Rapid Antigen Tests: FAQs Answered | BOOM | COVID19 Tests | COVID19 vaccine | COVID19 News

    3:12

    What is the difference between RT-PCR and Rapid Antigen Tests for #COVID19? Is a COVID-19 test painful? - and all other questions, answered here.

    Watch Dr Subhojit Sen, Scientist, Centre For Excellence In Basic Sciences, Mumbai, explain COVID testing in simple terms. #coronavirus #covid19 #COVID19Vaccine #COVID19News

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  • VERIFY: Are rapid antigen tests really less accurate than other COVID-19 tests? Whens the best time

    1:56

    As of December 7, 2020 the Food & Drug Administration has approved Emergency Use Authorizations for almost 300 different tests, included diagnostic and serology tests.

    About two-thirds of those tests are the traditional molecular tests, such as the PCR test, that can be run in a lab in a matter of hours but take a few days to get results.

    But only seven antigen tests, commonly referred to as 'rapid tests,' have been granted an Emergency Use Authorization. Those are the tests that can take as little as 15 minutes and can be run without a lab.

    For months, antigen tests have drawn skepticism and criticism from the public for producing false results.

    Elon Musk publicly questioned the accuracy of antigen tests on Twitter in November.

    Something extremely bogus is going on, he said. Was tested for covid four times today. Two tests came back negative, two came back positive. Same machine, same test, same nurse. Rapid antigen test from BD.

    Are antigen tests less accurate than other tests, like the PCR?
    Sources:

    Our Verify researchers asked this question to two infectious disease experts:

    Dr. Hanna Akselrod, assistant professor of medicine and co-director of the COVID-19 Recovery Clinic at the George Washington University
    Dr. Stuart Ray, professor of Medicine at Johns Hopkins University.

    We found out it's a difficult question to answer without defining the term, accurate.

    When you ask me whether antigen tests are accurate, are they accurate for what purpose?, Ray said. And I'll give you some choices.

    Here's the list Ray came up with:

    Are they an accurate measure of whether someone's infectious with SARS-CoV-2?
    Are they an accurate measure of whether someone has or recently has had SARS-CoV-2?
    Are they an accurate measure of whether or not a person's current symptoms are from a SARS-CoV-2 infection?
    Are they a good predictor of whether or not this person will be shedding the this virus tomorrow or the next day, because they had an exposure two days ago?

  • COVID 19 Antigen Testing At Home: Would It Be Too Expensive?

    2:47

    Harvard professor and epidemiologist Michael Mina, MD, PhD, explains the relative cost of frequent, at-home COVID-19 testing (using inexpensive paper strip antigen tests) to allow schools and businesses to re-open.

    Michael Mina, MD Ph.D. is an Assistant Professor of Epidemiology, Immunology, and Infectious Diseases at Harvard T. H. Chan School of Public Health. Dr. Mina is also a core member of the Center for Communicable Disease Dynamics (CCDD) and an Associate Medical Director in Clinical Microbiology. Dr. Mina's bio is here:

    We have no financial disclosures related to any medical products. MedCram has a strong interest in curbing this pandemic and safely re-opening schools and our economy, and we believe Dr. Mina's approach to testing could be effective - especially compared to the current testing approach in the United States.

    Other Q/A videos with Dr. Michael Mina on inexpensive quick paper antigen tests for COVID-19:

    Paper Strip Tests for COVID-19: Should We Wait Until They Rival PCR Tests?

    Are Antigen Paper Tests for COVID 19 Accurate Enough?

    False Positives and COVID 19 Rapid Tests:

    What if Some Don't Cooperate with COVID 19 Rapid Testing (Herd Immunity and Effects)?


    MORE VIDEOS AND RESOURCES ON AT-HOME COVID 19 SCREENING TESTS:

    Dr. Michael Mina is the director (on a volunteer basis) of where you can get more info and streamlined ways to contact your governor and representatives in Congress about at-home COVID 19 screening

    See our previous MedCram videos that have featured Dr. Mina's research about COVID 19 testing and how quickly identifying individuals who are contagious (and who have high levels of SARS-CoV-2 virus) should be the primary focus.

    A 3-minute explainer video on inexpensive at-home rapid screening tests by MedCram:

    A video summary and highlights of Dr. Mina's research and ideas in our MedCram COVID 19 Update 98:

    A 5 minute summary with Dr. Mina of some key differences between COVID-19 antigen tests with rapid results:

    A short explainer video on daily quick tests produced by Dr. Allen:

    Summary Article Featured in the Atlantic about Inexpensive Rapid Testing:

    Dr. Mina's research paper: Test sensitivity is secondary to frequency and turnaround time for COVID-19 surveillance (Pre-print)

    TWiV 640: Test often, fast turnaround, interview with Michael Mina:

    Visit MedCram.com for over 100 coronavirus pandemic updates including sensitivity vs specificity, covid 19 tests, diagnosis and treatment, and many more topics.

    ------------------------------------------------------
    Video produced by Kyle Allred, Co-Founder of
    Contact Kyle or MedCram by emailing: customers@medcram.com

    #COVID19 #rapidtestsnow #dailyquicktest

  • What is a false positive COVID test? And how do they happen? | ABC News

    6:11

    Two cases of COVID-19 previously linked to Melbourne's growing outbreak have been reclassified as false positives, the Department of Health has said.
    Subscribe: Read more here:

    ABC News provides around the clock coverage of news events as they break in Australia and abroad, including the latest coronavirus pandemic updates. It's news when you want it, from Australia's most trusted news organisation.

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    Watch more ABC News content ad-free on iview:

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    #ABCNews #ABCNewsAustralia

  • Making Sense of False Positives from Rapid Antigen Tests

    9:12

    False positives from rapid antigen tests pose a lot of questions. In this video, we review data that helps us answer these questions, and talk about the different antigen tests now available. For more information on #COVID19 testing and #medtech news, subscribe to my Youtube channel.

    ► Sign up for the monthly MedTech Quality Journal, for the most recent videos and articles to your inbox once per month:

    ► Are you a professional in Quality, Regulatory, Risk Management, Product Development, Medical Safety, or Clinical Affairs? Sign up for our free member's portal, where you will gain access to Knowledge Sharing Discussion Forum, monthly interactive Q&A sessions, workshops, blogs, videos, and more.

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    Data Source: FDA's MAUDE database
    Search using Product Code QKP

  • Ct value in your COVID-19 test? Why so important? What is it? Severe COVID-19? Isolation,Second wave

    3:31

    Many doctors are now telling patients that their Covid-19 test reports should mention the cycle threshold (Ct) value, and not just the positive or negative outcome.

    Ct value indicates the number of cycles needed in the RT-PCR test to amplify viral RNA, so it can reach a detectable level. A lower Ct value, 20 or below, is a sign of high viral load. Apart from ascertaining the severity of symptoms, doctors are also using Ct value to suggest home isolation or hospital admission to patients.


    This video will explain everything you must know about Ct value and how one must address it.

    #covid19 #vaccine #moderna #pfizer
    #sputnik #astrazeneca #mRNA #corona

    00:42 - What is Ct value in your COVID-19
    test report

    00:52 - How is Ct values measured

    01:54 - Understanding with an example

    02:11 - COVID-19 Report analysis

    02:26 - Why you shouldn't panic with your Ct scores

    02:45 - Avoiding false sense of security

    03:03 - How can Ct values turn significant

    || THANK YOU ||

    My Instagram handle:

  • COVID-19 DIAGNOSIS: MOST ACCURATE TEST? RT-PCR / ANTIBODY DETECTION TEST?

    11:00

    Hi. If you have cough or fever (either low grade or a high grade), with breathing discomfort or any other symptoms, then the only way to find out whether you have COVID-19 or not is to get tested.
    In response to the growing COVID-19 pandemic, multiple diagnostic test manufacturers have developed and begun selling rapid and easy-to-use devices.
    However, before these tests can be recommended, they must be validated in the appropriate populations and settings. Inadequate tests may miss patients with active infection or falsely categorize patients as having the disease when they do not, further hampering disease control efforts.

    Now the question arises, which test to get it done??
    Listen to this video till the end to find out about the different kinds of tests available, which diagnostic test is best and how each kind of diagnostic plays a role in the fight against COVID-19.

    Disclaimer: Information provided by INTELLECT MEDICOS is not meant to substitute for medical advise by any physician, or experienced health care professional. Always seek advise from your physician about the clinical condition and usage of particular treatment or therapy.

    REFERENCES:

    1. Liu Y, Liu Y, Diao B, Ren Feifei, et al. Diagnostic indexes of a rapid IgG/IgM combined antibody test for SARS-CoV-2. medxriv [Internet]. 2020;

    2. Zhang P, Gao Q, Wang T, Ke Y, et al. Evaluation of recombinant nucleocapsid and spice protein serological diagnosis of novel coronavirus disease 2019 (COVID-19). medxriv [Internet]. 2020;

    3. Pan Y, Li X, Yang G, Fan J, et al. Serological immunochromatographic approach in diagnosis with SARS-CoV-2 infected COVID-19 patients. medxriv [Internet]. 2020;

    5. Li Z, Yi Y, Luo X, Xion N, et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. Journal of medical virology.

    6. Zhao J, Yuan Q, Wang H, Liu W, Liao X, Su Y, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. medxriv [Internet]. 2020;

    7. Okba N.M.A, Muller M.A., Li W, Wang C, et al. SARS-COV-2 specific antibody responses in COVID-19 patients. medxriv [Internet]. 2020

    8. Gorse GJ, Donovan MM, Patel GB. Antibodies to coronaviruses are higher in older compared with younger adults and binding antibodies are more sensitive than neutralizing antibodies identifying coronavirus-associated illnesses. Journal of medical virology.

    9. Lin D, Liu L, Zhang M, Hu Y, et al. Evaluation of serological tests in the diagnosis of 2019 novel coronavirus (SARS-CoV-2) infections during the COVID-19 outbreak. medxriv [Internet]. 2020;

    10. Wölfel R, Corman V, Guggemos W, Seilmaier M, Mueller M, Niemeyer D, et al. Virological assessment of hospitalized patients with COVID-2019. Nature [Internet]. 2020;

    11. Lou B, Li T, Zheng S, Su Y, Li Z, Liu W, et al. Serology characteristics of SARS-CoV-2 infection since the exposure and post symptoms onset. medxriv [Internet]. 2020;

    12. Liu W, Liu L, Kou G, Zheng Y, et al. Evaluation of nucleocapsid and spike protein-based ELISAs for detecting antibodies against SARS-CoV-2. medxriv [Internet]. 2020;

    13. Zhang W, Du R, Li B, Zheng X, et al. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerging microbes and infections. 2020; 9(1):386-389.

    14. Zhou P, Yang XL, Wang X, Hu B, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020 Mar;579(7798):270-273. doi: 10.1038/s41586-020-2012-7. Epub 2020 Feb 3.
    15. Wang N, Li SY, Yang XL, et al. Serological Evidence of Bat SARS-Related Coronavirus Infection in Humans, China. Virol Sin. 2018;33(1):104–107. doi:10.1007/s12250-018-0012-7

    16. Che X, Qiu L, Liao Z, Wang Y, et al. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. The Journal of Infectious Diseases, Volume 191, Issue 12, 15 June 2005, Pages 2033–2037,

    17.
    You can connect with us on:

    1. Instagram page: intellect.medicos
    (we upload interesting animated mnemonics every day on our page)

    2. Facebook page. INTELLECT MEDICOS

    #COVID19 #RTPCR #CORONAVIRUS

  • What is RT-PCR test for Covid-19 and how does it work?

    8:13

    #PureScience #COVID19 #RTPCR
    COVID-19 is tested most popularly through the RT-PCR test, which is a genetic test that works on the RNA of the SARS-CoV-2 virus. How does this test work? ThePrint’s Sandhya Ramesh explains how samples are collected and processed to produce a result in an RT-PCR test.
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  • ‘Tested Negative Twice Via Rapid Test, RT-PCR Showed I Had COVID’ | The Quint

    2:49

    #MyReport: Priyam Dutta from Bengaluru was tested negative twice for #coronavirus via a rapid test despite showing symptoms of COVID-19 like loss of smell and taste. However, an RT-PCR test showed that he was coronavirus positive.

    Support The Quint's independent journalism. Become a member now:
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  • The 4 Types of COVID-19 Tests and What Each One Tells Us || Decode || Factly

    3:14

    There are four types of tests that can help us detect if we are carrying or have ever carried the novel coronavirus. Watch this video to know what each test does in a nutshell.
    #CoronavirusTests #Decode #Factly

    For detailed reading, please go through the following:

    What are the different types of tests being used in India for COVID-19 detection?:
    How is the COVID-19 Virus Detected using Real Time RT-PCR?:

    Newer Additional Strategies for COVID-19 Testing:
    Revised Guidelines for TrueNat testing for COVID-19: on Use of Rapid Antigen Detection Test for COVID-19:

    Modern life comes with many complications. To help you navigate through the labyrinth of rules in this age and also to make you aware of your rights as a citizen of India, we bring you Decode by Factly.

    Write to us with your suggestions and feedback ► hi@factly.in

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  • Covid-19 News: Is A Positive Covid Test Really Positive? The Importance Of CT Value

    10:51

    Coronavirus: Facts Vs Myths | Many health experts and doctors are raising a new concern over coronavirus testing. They claim that the standard tests are diagnosing huge numbers of people who may be carrying relatively insignificant amounts of the virus. The PCR test amplifies genetic matter from the virus in cycles; the fewer cycles required, the greater the amount of virus, or viral load, in the sample. The greater the viral load, the more likely the patient is to be contagious. This number of amplification cycles needed to find the virus, called the cycle threshold, is never included in the results sent to doctors and coronavirus patients, although it could tell them how infectious the patients are.

    NDTV is one of the leaders in the production and broadcasting of un-biased and comprehensive news and entertainment programmes in India and abroad. NDTV delivers reliable information across all platforms: TV, Internet and Mobile.

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  • CT value in rt-PCR Report in covid-19 patients...

    16:48

    #DURecorder
    This is my video recorded with DU Recorder. It's easy to record your screen and livestream. Download link:
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  • DNS: RAPID ANTIGEN TEST

    5:48

    DOWNLOAD PDF:
    As the number of Corona cases are increasing in INDIA, the speed of testing is also increasing with it. There a number of testing facilities on which the ICMR relies on for detecting coronavirus in humans. ON June 15, the ICMR approved one more test for corona virus diagnosis. This new test is known as Rapid Antigen test. This will be used in specified settings and the kits of the same is allowed to be manufacture by only one manufacturer for now.

    In this Dns we will now about Rapid Antigen test.

    Voice and Script : Shiba
    Graphics : Pankaj Jain
    Editor : Shailesh Kumar

  • Testing for COVID 19 using RT PCR

    10:04

    In this video from our COVID Mini: Diagnostics course, you'll take a deep dive into the RT-PCR process for SARS-CoV-2; learning about how it works, how it can go wrong, and an important value you should look for that may help you predict disease progress.

    Continue your understanding of this deadly disease through our COVID Mini: Diagnostics course.

    Check out our course library and register for a free trial account:

    Please Note: Medmastery's videos, medical lectures, medical illustrations, and medical animations are for medical education and exam preparation purposes, and not intended to replace recommendations by your doctor or health care provider.

    #medmastery #coronavirus #COVID19 #sarscov2 #coronaviruschina #coronavirustruth #WHO #wuhan #infection #pandemic #publichealth

  • Coronavirus Test: Real time RT-PCR - Animation video

    8:20

    I make animations in biology with PowerPoint, this animation video is about the standard coronavirus test, real time RT-PCR method, which is a laboratory technique combining reverse transcription of RNA into complementary DNA, and amplification of specific DNA targets using polymerase chain reaction (PCR).

    Coronavirus Antibody Test - Animation video :


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    if you like this animation video hit like and subscribe for more.

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  • COVID 19 TESTS | RT-PCR Vs Rapid Antigen Test | कोनसा कोविड टेस्ट करवाए | सबसे अच्छा कोविड टेस्ट

    5:54

    Dr.Maulik Shah describes the basics of all COVID TESTs especially comparing RT-PCR Vs Rapid Antigen Test in the Hindi language.
    Disclaimer: This program is meant for general knowledge updating only and not to be taken as medical advice of any form. Consult your doctor for any medical complaints OR Emergency.

    #RT-PCR #RapidAntigenTest #COVID19 #Tests

    Watch our other videos on the COVID topic….
    1. Role of CT Scan in the diagnosis of COVID19 (Hindi):
    2. COVID 19 TESTS – RT-PCR Vs Rapid Antigen Test (Hindi):
    3. Use of Pulse oximeter in COVID patients (Hindi):
    4. Corona, Children & Schooling (Gujarati):
    5. Routine Immunization of Children in Corona Pandemic (Hindi):
    6. Is wearing the mask really effective (Hindi):
    7. Tips on wearing gloves in daily life(Hindi) :
    8. N-95 Respirator (Hindi):
    9. Triple Layer Mask (Hindi):
    10. Personal Protective Equipment (Gujarati):
    11. Triple Layer Mask (Gujarati):
    12. N-95 Respirator (Gujarati):
    13. Tips on protecting self from ‘Airborne Infection’ (Gujarati):
    14. Fight against Corona as a nation (Gujarati):
    15. How to protect self from CORONA infection (Gujarati):

    Also, follow our other videos on other topics
    1. Childcare videos:
    2. Newborn care videos:
    3. Pregnancy care videos:
    4. General health videos:
    5. Bachcha Party Bindaas Videos:
    6. 1000 Days Videos:
    7. MR Vaccination Campaign Gujarat Videos:
    8. Medical Education Videos:
    9. Interviews : મળવા જેવો માણસ:

  • Explained: Why Govt Insists On RT-PCR Test Instead Of Rapid Antigen Test For Screening For COVID-19?

    3:45

    Explained: Why Govt Insists On RT-PCR Test Instead Of Rapid Antigen Test For Screening For COVID-19?..,

    ► TV9 Kannada Website:
    ► Subscribe to Tv9 Kannada:
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    #TV9Kannada #RTPCR #RapidAntigenTest #CovidTest #CoronavirusTest

  • BBMP in doubt as Rapid antigen test says COVID positive, but RT-PCR test says negative

    4:49

    BBMP in doubt as Rapid antigen test says COVID positive, but RT-PCR test says negative..,

    ► TV9 Kannada Website:
    ► Subscribe to Tv9 Kannada:
    ► Like us on Facebook:
    ► Follow us on Twitter:
    ► Download TV9 Kannada Android App:
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    #TV9Kannada #RapidAntigenTest #BBMP #RTPCR

  • RT PCR test for Covid 19| what is ct value?

    6:14

    This Video lecture of BiologyExplained talks in detail about what is ct value in Covid Test.
    To understand what is ct value one has to perform RT PCR, which in case of Covid means Real Time Reverse Transcription Polymerase
    Chain reaction. Polymerase chain reaction is a process of amplification of small amount of DNA to a large amount. And it is done
    with the help of an enzyme DNA polymerase. Reverse Transcription is the process by which cDNA is made from RNA.
    And real time means we are observing the values instantly as the reaction is going. Ct value in case of RT PCR means
    cycle threshold value that is at what cycle the DNA amount becomes detectable. If the Viral DNA (Sars-CoV2 Virus has RNA which is
    converted to DNA by reverse transcription) is detectable before cycle 35 that is if ct value is below 35 then one is Covid positive.
    Lesser the ct value more is the viral load. That means if a person have ct value of 17 he/she is positive. Again if a person has
    ct value of 25, then also he/she is positive. But the person with lower ct value has higher viral load.
    However please remember ct value indicates infectivity of the disease and not severity.
    LINK for English version:
    LINK for Bangla version:

    Time strap: English
    00:00 Introduction
    00:33 What is RT PCR
    03:30 What is ct value
    04:49 ct value and Covid 19

    #ctValue #RTPCR #COVID #BiologyExpalained

  • FORA COVID-19 Nasal Antigen Test - Product Demonstration

    3:29

    Purchase online from:

    The FORA COVID-19 Antigen Rapid Test is clinically validated for testing samples collected from the front area of the nose instead of the nasopharynx. This results in a simplified and more comfortable testing experience. Find our more by visiting the link above.

  • Our COVID-19 testing system is BROKEN - Heres How to FIX It!

    7:41

    Dr. Christy explains at-home rapid antigen testing as a public health screening tool and ways to get involved to get this technology available.

    References:


    Cycle threshold article:
    NYT article:

  • COVID-19 ECHO Session #28: Manufacturers of COVID-19 Rapid Diagnostics Tests: Abbott Panbio

    1:5:59

    The global partnership to launch the secure availability of antigen rapid tests was recently announced by WHO, The Global Fund, Unitaid, Africa CDC, FIND and other partners. The aim of this partnership is to ensure that low-priced, high-quality antigen rapid tests are made available internationally in order to avoid the test shortages experienced since the start of the pandemic. Antigen tests provide a lower cost and more flexible way to deploy testing programs in health facilities, in communities, for vulnerable populations and other settings for effective epidemic control. WHO has recently released its initial guidance recommending the use of SARS-CoV-2 antigen rapid tests and has also added new antigen tests to its list of SARS-CoV-2 tests approved for emergency use. One of these tests is the Abbott Panbio rapid antigen test. Participants will learn about:

    • Details on the Panbio rapid antigen test and how it works
    • Status regarding regulatory approval
    • Performance, run time & other technical considerations
    • Requirements/consumables not supplied with the test(s)
    • Sympheos data system that works with the test
    • How antigen tests can be deployed to support testing programmes for COVID-19 epidemic disease control

    Presenter and Panelists:
    • Dr Kuku Appiah, Director, Medical & Scientific Affairs, Africa, Abbott Rapid Diagnostics
    • Jose Bermudez, Technical Services and Sympheos, Abbott, Abbott Rapid Diagnostics
    • Dr Evans Mathebula, Scientific Affairs Manager, Abbott, Abbott Rapid Diagnostics

    Please note that ASLM does not endorse specific manufacturers over others. This session is for informational purposes only.

  • COVID-19 ECHO session #31: Manufacturers of COVID-19 Rapid Diagnostics Tests: LumiraDx

    52:04

    This session, hosted by ASLM, focuses on the LumiraDx SARS-CoV-2 antigen assay, one of a variety of SARS-CoV-2 antigen tests. The global partnership to launch the secure availability of antigen rapid tests was recently announced by WHO, The Global Fund, Unitaid, Africa CDC, FIND and other partners. The aim of this partnership is to ensure that low-priced, high-quality antigen rapid tests are made available internationally in order to avoid the test shortages experienced since the start of the pandemic. Antigen tests provide a lower cost and more flexible way to deploy testing programs in health facilities, in communities, for vulnerable populations and other settings for effective epidemic control. In this session, participants will learn about the following:

    · Details about the LumiraDx antigen test platform and how it works
    · Status regarding regulatory approval
    · Performance, run time and other technical considerations
    · Requirements/consumables not supplied with the test(s)
    · How antigen tests can be deployed to support testing programmes for COVID-19 epidemic disease control

    Presenter:
    Dr Nigel Lindner, Chief Technology Officer, LumiraDx

    Please note that ASLM does not endorse specific manufacturers over others. This session is for informational purposes only.

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